FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007929793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397089
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT53201.3397500308495074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51191.2891316556238022No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17940.45178788634286016No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16640.4190496337093196No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC15980.40242867467998356No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG15550.39159986803965857No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12880.32436053378461754No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12090.3044657494919275No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11530.29036311758824845No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC11020.2775196492473979No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10240.2578766976672736No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10080.25384737426622245No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9730.24503322932642305No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT9320.23470808811122948No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8710.21934629264472194No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8430.21229497669288244No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8420.21204314398031676No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8210.20675465701643714No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC7170.18056405490960464No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7140.17980855677190755No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT6890.17351273895776514No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6810.17149807725723956No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6560.16520225944309713No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6550.16495042673053142No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5970.150344129401721No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5960.15009229668915533No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC5550.13976715547396176No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5520.13901165733626467No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5510.13875982462369896No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5270.13271583952212224No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5090.1281828506959397No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG4670.11760587676818045No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG4620.11634671320535195No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4450.1120655570917351No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4440.11181372437916941No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4330.10904356454094674No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4070.10249591401423862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)