FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462891
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT55791.2052513442689532No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29590.6392433639884983No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24190.5225852306482519No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20220.43681989928514486No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19600.4234258173090425No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA17130.37006552298489276No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15970.3450056276747658No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15890.343277359032688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14730.31821746372256104No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12570.2715542103864625No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10940.23634073680412884No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10680.23072386371737622No Hit
CTGAACACCTCTTACCAGAGCCAGCTGATCA10450.22575509137140276No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10120.21862598322283217No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10030.2166816810004947No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA9730.21020067359270325No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8990.1942141886534843No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA8620.18622094618387483No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC8610.1860049126036151No Hit
GCTGCCGCGTACAACCCGCACCACCTGCCTC8300.1793078716155639No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8270.17865977087478477No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7340.15856864791063124No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7060.15251970766335918No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6990.15100747260154118No Hit
ATGTCTCTGGCAACTGATATCCTGAAACACG6610.142798196551672No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC6530.14106992790959427No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6500.14042182716881513No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG6350.1371813234649194No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6110.13199651753868621No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6070.13113238321764734No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5840.1261636108716739No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5680.12270707358751844No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5650.12205897284673929No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5250.11341762963635069No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5230.11298556247583125No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5200.11233746173505209No Hit
GAAGCGCAGAAACTGAACGACTCTCAGGCCC4860.10499232000622176No Hit
GCAGTTTCTGTTGACTGCTCTGAATACCCGA4810.10391215210492318No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT4800.10369611852466347No Hit
GCTCAGCACGATGAAGCACAGCAGAACGCGT4770.10304801778388434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)