FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491752
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT35420.7202817680456816No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20070.4081325546210285No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19800.40264198213733754No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19520.3969480551172136No Hit
ACTCTGACCGGTAAAGTTGAGGATAACCACG13590.2763588150124453No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13200.2684279880915583No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11270.2291805625599896No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11270.2291805625599896No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11240.2285704989506906No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11010.22389334461273164No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG10230.20803169077095773No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10140.20620149994306072No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9400.1911532642470188No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9230.18769623712765784No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8810.1791553465974719No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8580.17447819225951294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8460.17203793782231694No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7910.16085343831850202No Hit
GCGAAACTGCTGTATAACAACCTGGATGCAT7480.15210919325188307No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7300.14844881159608908No Hit
AACCTGGATGCATTCGGTATTATGGATTACA7170.1458052026224601No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7110.14458507540386212No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6970.14173811189380012No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6800.13828108477443915No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6590.1340106395093462No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6200.12607981258845924No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6130.12465633083342824No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6120.12445297629699523No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6020.12241943093266525No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5400.10981144967381933No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5360.10899803152808732No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5350.10879467699165433No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5170.10513429533586034No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5080.10330410450796336No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5070.10310074997153036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)