FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504131
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA81451.6156514874110104No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA41670.8265708714599975No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA41500.8231987320755915No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39370.7809478092003864No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT28810.5714784450866938No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25310.5020520459959812No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24460.485191349073951No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17510.34733035659382183No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15460.30666632284069023No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12500.24795142532397335No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG12490.24775306418371415No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12300.24398420251878974No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11340.2249415330539086No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC10700.21224642007732114No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10460.20748575271110087No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8780.17416108114755888No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8740.17336763658652216No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8670.1719791086047079No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8650.17158238632418954No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8030.15928399562812046No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7720.15313480028008591No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7270.1442085489684229No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7180.14242329870609027No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6760.1340921308152048No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6610.13111671371131708No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6590.13071999143079874No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6010.11921504529576636No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5980.11861996187498883No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5850.11604126705161953No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5650.11207404424643595No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5560.11028879398410335No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5530.1096937105633258No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5480.10870190486202991No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5460.10830518258151155No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5390.10691665459969729No Hit
GTTCTGGTTAACGAAGGTAACGGTTATGTGG5350.10612321003866057No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5270.10453632091658716No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5250.10413959863606881No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG5190.10294943179451371No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5150.10215598723347702No Hit
CCTACCGATGGCCCGCCGCACCGTGGTGGCG5060.10037073697114442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)