FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397203
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA28910.7278394171242413No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25190.6341845353635295No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC20760.5226546627291334No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20270.510318401421943No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20010.5037726301160868No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18510.46600856488999326No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG16760.4219504887928842No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16630.41867760313995617No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15280.38468994443647203No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15170.3819205796532252No Hit
GTAGTTCGGGTCGATATCTGCGACACCTTCGGCGTCCAGCATTT14760.37159840182475967No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11530.2902797813712384No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10490.2640966961478136No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9790.24647346570896997No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9100.229101995704967No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8390.21122700483128276No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG8350.21021996309192026No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8050.20266715004670158No Hit
GATACCGTCGGTCGGAACGAACGGCGTGCCTTTTTCGAAATATT7700.19385553482727974No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7690.19360377439243914No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG7670.1931002535227579No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7450.1875615239562642No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7170.18051223178072673No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7130.17950519004136425No Hit
GCCCTCAACATCCAGCATTTCCATGTCGCCCAGGGTAAACTTAG6950.17497350221423302No Hit
GTTACAGGTTACAGTCAGCGCTTTGTTAACATCAGAACCGGTCA6950.17497350221423302No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT6900.1737147000400299No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6350.15986787612379563No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6180.15558794873150505No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6110.1538256256876207No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6060.15256682351341758No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG5790.14576929177272074No Hit
GTAGTCATAGTCACGCGGCAGCAGTACACGTGCGGTAACACGCT5520.1389717600320239No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5420.13645415568361768No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5170.13016014481260213No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4990.1256284569854709No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4970.12512493611578968No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4730.11908268567961472No Hit
GCCGTTACCAGCGTACAGCAGAGACACTTTTTCCGGTTCGATAC4720.1188309252447741No Hit
GTCGCCATACAGCAGGGAAACTTTTTCCGGCTCGATGTTGGAGT4430.11152987263439601No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4320.10876050785114916No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC4220.10624290350274293No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG4110.10347353871949608No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4080.1027182574149742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)