FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397203
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT67291.6940959660425525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55141.3882070377111957No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA43521.0956614124263915No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22560.5679715410004456No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA20500.5161088914232772No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19600.4934504522876212No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19310.4861493996772431No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18640.4692814505429214No Hit
GAACCGATCTACGCTACCATCGACGACCTGG17440.4390701983620466No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15190.38242410052290643No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA15050.3788994544351377No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12140.3056371678965164No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11890.2993431570255008No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA9790.24647346570896997No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9320.2346407252714607No Hit
CTGCCGATCCAGCAGGTCAGCAAAATGGGCG8810.22180094309458892No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8700.21903157831134204No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8070.20317067091638283No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG7930.1996460248286141No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7800.196373139175686No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7710.19410729526212037No Hit
AACACCGATGCACACGCTTCCAGCGATGCCC7690.19360377439243914No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG7040.17723934612779862No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG6960.17522526264907365No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6420.16163019916768No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6340.159616115688955No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6260.15760203221023003No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5900.14853865655596762No Hit
ACTGCACTGTTCGCTCGCGAATACCAGACCC5750.14476225003335824No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5210.1311671865519646No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5110.12864958220355838No Hit
GAACGTGGTAACTTCTCCAAATTCACCCTGG5070.1276425404641959No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5010.12613197785515215No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5010.12613197785515215No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5000.12588021742031152No Hit
ACCCTGGGCGACATGGAAATGCTGGATGCAG4480.11278867480859912No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4160.10473234089369919No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4120.10372529915433669No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4110.10347353871949608No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA4050.10196297611045234No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4030.10145945524077109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)