FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465869
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT121392.6056681170028484No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA90751.9479724987067177No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA47921.028615340363922No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA46691.0022130684806243No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG36210.7772571259302508No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC29470.6325812621144571No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC19170.41148906666895635No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17510.3758567322573513No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC16220.3481665446724294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15350.3294917669988774No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC14270.3063092843696404No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13920.29879644277683215No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13400.28763450669608837No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12630.2711062551919102No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11880.2550073089216067No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11320.2429867623731135No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA10780.231395521058495No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10640.22839038442137166No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10570.22688781610281003No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10000.2146526169373794No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9820.21078886983250658No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9120.19576318664689002No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9080.19490457617914048No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9050.19426061832832833No Hit
GACAAAGCGGAAGCTCAGAGCACCCCGGAAC8360.17944958775964917No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA7780.16699973597728118No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC7720.1657118202756569No Hit
GACAAAGCGGAGGCCCAGTCTACCCCGGAAC7420.1592722417675355No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC7370.1581989786828486No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7160.15369127372716365No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG7160.15369127372716365No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG6820.14639308475129276No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA6610.14188537979560778No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6320.13566045390442377No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6090.13072344371486405No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6080.13050879109792668No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6070.13029413848098928No Hit
GCTGAAGCGCAGTCTACCCCGGAGCGTCCGG5900.12664504399305382No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5660.12149338118655675No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5500.11805893931555868No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5460.11720032884780915No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5300.11376588697681107No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5240.1124779712751868No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5130.11011679248887563No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5080.10904352940418872No Hit
GAAGCCGAGCTGGTTAACGACACCACCGCTC5060.10861422417031397No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT4960.10646769800094018No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC4750.10195999304525522No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA4720.10131603519444307No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4690.10067207734363094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)