FastQCFastQC Report
Wed 31 May 2023
EGAF00007929919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450634
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74191.6463471464647585No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA41490.9207028320100126No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37900.8410372941233906No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26010.5771868079195089No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT25430.5643160524949293No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19380.4300607588419871No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17630.39122658299196245No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16170.3588277848542276No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15320.3399654708699299No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15120.33552727934421284No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14100.3128925025630556No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12530.27805269908617636No Hit
TCCTACACCAACGGCAAACTGACTATCACCC11520.255639831881305No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11350.2518673690844455No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9760.21658374645499454No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8750.19417087925012316No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG8430.1870697728089758No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG8200.18196585255440115No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7910.17553047484211134No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7820.17353328865553863No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG7670.1702046450112508No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7270.16132826195981662No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7090.15733388958667122No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7040.15622434170524196No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6860.15222996933209654No Hit
GAAGCAGAAAGCCTGCGTGATGTTATTCGTA6460.14335358628066236No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6450.14313167670437651No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6280.13935921390751696No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6200.13758393729723012No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5930.13159237873751206No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC5680.12604463933036567No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5460.12116262865207685No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5140.1140615222109295No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5010.11117669771921337No Hit
GGTTACTACCAGCTGCTGCAGGCCTCTCTGA4990.11073287856664166No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4760.105628958312067No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA4640.10296604339663673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)