FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007929925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386804
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63041.6297659796692898No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25480.6587315539653158No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20840.5387741595226523No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16090.41597294753932224No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15880.41054384132532235No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14870.3844324257246564No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14130.36530128954199026No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13130.3394484028086576No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12900.3335022388599911No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12510.32341961303399136No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG11340.2931717355559922No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10150.26240680034332636No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9910.2562021075273265No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA9060.23422715380399375No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8910.23034922079399386No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8210.21225220008066098No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7800.2016525165199946No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7510.19415517936732815No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7490.1936381216326615No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7470.19312106389799485No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7290.188467544285995No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT7170.18536519787799505No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7010.18122873600066183No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6960.17993609166399518No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6420.16597553282799557No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6420.16597553282799557No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT6290.16261465755266233No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6270.16209759981799568No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6210.1605464266139957No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6190.16002936887932906No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5730.14813704098199607No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5530.1429664636353295No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5510.14244940590066288No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5370.13883000175799629No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5240.13546912648266304No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5220.13495206874799642No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC5100.13184972233999648No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC5020.12978149140132986No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4820.12461091405466333No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA4800.1240938563199967No Hit
CATCGTTCCCCACTGTGTCGTGATGAAGACG4720.12202562538133008No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA4710.12176709651399677No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC4680.12099150991199677No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC4620.11944033670799681No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4530.11711357690199688No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4390.11349417275933031No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC4340.11220152842266366No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4310.11142594182066369No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4280.11065035521866372No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT4260.11013329748399707No Hit
GACTTCGCTCATCAGTGCCTGCAGGCGGCAA4200.10858212427999711No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4040.10444566240266388No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4010.1036700758006639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)