FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386279
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA66771.7285433585568981No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60871.5758040173035552No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA33340.863106718201093No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA29160.7548947781266908No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT24070.623124736265756No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC24010.6215714548292814No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23430.6065564009433596No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG22050.5708309279044421No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG21190.5485672273149719No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC20890.5408008201325984No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG20850.5397652991749486No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC20470.5299278500772757No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20070.5195726405007779No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16920.4380253650858576No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC15770.4082541375534264No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12110.3135039699284714No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11920.3085852453796349No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11240.29098138909958865No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10140.26250456276421963No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9830.2544792753424338No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9480.24541846696299824No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9240.23920534121709955No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC9080.2350632573865004No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8600.22263700589470306No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT7890.20425650889641941No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7190.18613489213754825No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7170.18561713165872337No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG7020.18173392806753666No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA6800.17603856280046287No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6770.17526192208222555No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6310.16335343106925307No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5850.15144494005628056No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5820.1506682993380432No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5610.14523181431038187No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5580.14445517359214455No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5570.1441962933527321No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC5240.1356532454521214No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC5170.13384108377623427No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5100.13202892210034714No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5100.13202892210034714No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4850.12555691611503603No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC4830.12503915563621112No Hit
AACACCCAGGAACGCATGGAGCTGCGTAAAC4700.12167371252384934No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG4630.11986155084796223No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4590.11882602989031245No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4510.11675498797501288No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4250.1100241017502893No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4150.10743529935616486No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA3960.10251657480732838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)