FastQCFastQC Report
Sun 21 May 2023
EGAF00007929968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520901
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA32940.6323658430296736No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC31470.6041455094154168No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA30060.5770770261527622No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA25530.4901123246067871No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA20750.3983482465958023No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19610.3764630899153582No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT16230.3115755201084275No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15490.2973693657719989No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA15450.29660146553759736No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT12850.24668795030149682No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG11950.22941019502746204No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT10630.204069487292211No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10560.20272566188200827No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC10090.19370283412779013No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG10090.19370283412779013No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9820.1885195075455797No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9040.17354545297474952No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8680.16663435086513562No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8440.1620269494587263No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8380.160875099107124No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7040.13515044125467218No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7010.13457451607887103No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6600.12670353867625517No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6440.123631937738649No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6430.1234399626800486No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6300.12094428691824359No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT6170.11844861115643855No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6160.11825663609783817No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6080.11672083562903508No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6060.1163368855118343No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5720.10980973351942115No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5710.10961775846082078No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5630.10808195799201768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)