FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007930025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497190
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA94861.9079225245881855No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA53141.068806693618134No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC52131.0484925280074016No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49460.9947907238681389No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA49370.9929805506949054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44620.8974436332186889No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37690.7580602988797039No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA31890.6414046943824293No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG28290.5689977674530863No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT25080.5044349242744223No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22560.45375007542388224No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC21030.4229771314789115No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG19410.3903940143607072No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18920.38053862708421327No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13770.2769564955047366No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13630.2741406705685955No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13260.2666988475230797No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12780.25704459059916734No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12580.25302198354753713No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12280.24698807297009193No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11820.23773607675134253No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11270.22667390735935958No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10160.20434843822281218No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC10130.2037450471650677No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10010.2013314829340896No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9720.19549870270922587No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9420.1894647921317806No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9180.1846376636698244No Hit
CAGCGTCTGAAGGGTAAAGAAGGTCGTGTAC9110.18322975120175386No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9100.18302862084917235No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA8970.18041392626561276No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8890.17880488344496068No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8860.17820149238721614No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7830.15748506607132082No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7740.15567489289808725No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7670.15426698043001671No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7040.14159576821738168No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7030.14139463786480017No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6150.12369516683762748No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5940.1194714294334158No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5850.11766125626018221No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5630.11323638850338905No Hit
AGCGTGCACATCAAGATCCCGAAAGTTTCCC5580.11223073674048151No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5140.10338100122689516No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5030.10116856734849856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)