FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007930028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493003
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25610.5194694555611223No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18860.38255345302158406No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15340.3111542931787433No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG15020.3046634604657578No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13840.2807285148366237No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13140.26652981827696787No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12520.2539538298955584No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11100.2251507597316852No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10580.21460315657308374No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10400.21095206317202939No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10320.209329354993783No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9770.19817323626833916No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8800.17849789960710177No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8650.1754553217728898No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8490.17220990541639705No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8390.1701815201935891No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7750.15719985476761805No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7490.1519260531883173No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7420.15050618353235173No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6700.1359018099281343No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA6690.13569897140585352No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6340.1285996231260256No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA6330.1283967846037448No Hit
AGAACGGGTCAGATCAATCAGGGACATGTCGCAGTGGCGACAGA6200.12575988381409445No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6180.12535420676953285No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6150.12474569120269047No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5730.11622647326689696No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5680.11521228065549297No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5400.10953280203163064No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5390.10932996350934984No Hit
AGACAGGCTTTTACGAGTGGACGCAACCTGTTTACCGACAGCGT5120.10385332340776832No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG5030.10202777670724113No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT4990.10121642261811793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)