FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007930069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509183
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA71191.3981220897005595No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35970.7064257840501352No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA32730.642794437363384No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29680.5828945585378931No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC26020.5110147039473039No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG25920.509050773494009No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG25080.49255375768633275No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG21790.4279404457729343No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT17920.351936337230426No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16770.32935113701753593No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA15800.3103010116205765No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15800.3103010116205765No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15140.2973390706288309No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC15060.2957679262661951No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14580.2863410600903801No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14300.28084205482115465No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA13720.2694512581920449No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11990.2354752613500451No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC11250.22094217599566363No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA10520.20660548368661172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10070.19776779664678512No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA10030.19698222446546723No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9730.19109043310558288No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA9670.189912074833606No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9660.1897156817882765No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA9370.18402028347372162No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9260.18185995997509738No Hit
GAACCGATCTACGCTACCATCGACGACCTGG9100.17871767124982568No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8800.17282587988994133No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8660.17007637725532865No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8580.1685052328926928No Hit
GAAGTTGGCCGTAAACGTGTATTCCAGGAAA7900.15515050581028825No Hit
CGTGGCGAATTTGAACTGGTTGCGCAGCGCA7870.1545613266742998No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA7550.14827674922375647No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7300.14336692309051952No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7060.13865349000261204No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7020.13786791782129412No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA6940.1362967734586583No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG6620.13001219600811495No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6600.12961940991745602No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6570.12903023078146758No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6530.12824465860014964No Hit
GATTACGGTAAGAAACAGGACAAAGCCCTGG6490.12745908641883175No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6370.125102369874878No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6360.1249059768295485No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6360.1249059768295485No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6350.12470958378421904No Hit
GAACGTGTCGACATGCTGAAGGCGGGTGCAA6340.12451319073888956No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC6220.12215647419493582No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG6130.1203889367869705No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6050.11881779242433467No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5880.11547911065373352No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5850.11488993151774508No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5640.11076567756582603No Hit
GTCGGTGACGGTGAGCCAACGACCGATGCTA5600.10998010538450813No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5460.10723060274989542No Hit
CGTACCGTTGAATATTCCCCATTCGCTGGTC5440.10683781665923647No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5360.10526667229660064No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5220.10251716966198793No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5190.10192799052599949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)