FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007930070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492804
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC30950.6280387334518389No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT30620.6213423592340972No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC23430.4754425694596635No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG23210.470978319981169No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21230.43080007467471854No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG19940.40462333909627357No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG19770.40117369177198237No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT18520.37580863791690006No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG16780.3405004829506254No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT15410.3127003839254551No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15160.30762737315443867No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14540.29504630644231783No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14150.2871324096395322No Hit
GTAGTTCGGGTCGATATCTGCGACACCTTCGGCGTCCAGCATTT14050.28510320533112554No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT14020.2844944440386036No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13290.2696812525872355No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG12840.26054983319940583No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12280.24918628907232895No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT11750.23843150623777404No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA11570.2347789384826422No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11030.2238212352172466No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG10520.21347229324437303No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT10360.2102255663509225No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG9430.19135396628274123No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9170.18607803508088408No Hit
GTAAGCCAGTACCGCGCGACGGTTTTTGGAGTATGCCGCTTCGT8680.17613493396969182No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG8650.17552617267716983No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG8540.17329404793792258No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8390.1702502414753127No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT8370.16984440061363137No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG8060.16355386725757098No Hit
ACGCTGCAGACCGCTGCGACGGTGAATGGCACGGCGCGGTGCTG8010.16253926510336766No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7830.1588866973482358No Hit
GTATGCCAGTACCGCACGACGGTTTTTGGAGTAGGCAGCTTCAT7100.1440735058968677No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT6670.1353479273707194No Hit
TACCGGGTGACCAGCCGGGATGACCACAACGTCACCCGGGGACA6640.13473916607819741No Hit
AACACCGTTGGTGGTACCAACACCTTTGTCCGGAGATTTGGATG6620.13433332521651611No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG6490.13169535961558754No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6450.1308836778922249No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6450.1308836778922249No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6270.12723111013709304No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6160.1249989853978458No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT6080.12337562195112053No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5970.12114349721187329No Hit
GCCCTCAACATCCAGCATTTCCATGTCGCCCAGGGTAAACTTAG5940.12053473591935131No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5850.11870845204178537No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5830.11830261118010406No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5800.11769384988758208No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5780.11728800902590075No Hit
GGTTTCTGCAGCCGCGTTGTAGGTCAGAGAGGCGGACAGCTGGT5670.11505588428665352No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5500.11160623696236233No Hit
TTCATCACCATCACCGCCTTCGTCACCGTCATCGCCGTCATCGC5310.1077507487763898No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC5230.10612738532966454No Hit
CAGCACGACTTTAGACGCTTTGTTACCGTATTTAACAACATAGG5170.10490986274462057No Hit
GGTCGCACCAGCTTCCAGTTCCAGGGTAATGTTGGATTTCAGGT5030.10206897671285134No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4960.10064853369696676No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4930.10003977240444478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)