FastQCFastQC Report
Sun 21 May 2023
EGAF00007930071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492804
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA67731.3743800780837818No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45540.92409964204836No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA33260.6749133529760311No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31240.633923425946218No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28290.5740618988482237No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG25550.5184617007978831No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC25060.5085185996866909No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24020.4874148748792623No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG21120.42856794993547126No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT17150.3480085388917298No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15350.3114828613404112No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14460.2934229429955926No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC14360.291393738687186No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14170.28753825050121345No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA14120.28652364834701016No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT14030.2846973644694442No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13410.2721162977573234No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA12950.2627819579386531No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11760.2386344266686147No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC10910.2213861900471587No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA10100.20494963514906533No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9670.19622405662291703No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9540.19358609102198845No Hit
GAACCGATCTACGCTACCATCGACGACCTGG9380.1903393641285379No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA9310.1889189211126533No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA9070.1840488307724775No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA8620.17491741138464786No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8440.171264843629516No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8390.1702502414753127No Hit
GAAGTTGGCCGTAAACGTGTATTCCAGGAAA8000.162336344672527No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7510.1523932435613347No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7490.1519874026996534No Hit
CGTGGCGAATTTGAACTGGTTGCGCAGCGCA7310.14833483494452157No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA6890.13981217684921388No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA6870.13940633598753258No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6850.13900049512585125No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6830.13859465426416992No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6640.13473916607819741No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6410.13007199616886228No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6390.12966615530718095No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6380.1294632348763403No Hit
GATTACGGTAAGAAACAGGACAAAGCCCTGG6360.12905739401465896No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6340.12865155315297766No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6290.12763695099877434No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6010.12195517893523593No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5990.1215493380735546No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG5820.1180996907492634No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5740.11647632730253812No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5720.11607048644085682No Hit
CGTACCGTTGAATATTCCCCATTCGCTGGTC5600.1136354412707689No Hit
GAACGTGTCGACATGCTGAAGGCGGGTGCAA5600.1136354412707689No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5530.11221499825488429No Hit
GTCGGTGACGGTGAGCCAACGACCGATGCTA5370.10896827136143375No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5250.10653322619134586No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG5190.1053157036063019No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC5190.1053157036063019No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5050.10247481757453267No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5010.10166313585117004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)