FastQCFastQC Report
Wed 31 May 2023
EGAF00007930304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453907
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25710.5664155873339692No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA23930.5272005058304895No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19750.43511115713130666No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG17740.39082895835490533No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16040.3533763524246156No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG15370.33861561949914853No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG14720.32429550546697894No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG14150.31173786700799944No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG13620.300061466335615No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG12890.28397887673025535No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG12700.2797929972439288No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG11760.2590839092589451No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG11470.25269493530613096No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11070.24388255744018048No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9350.20598933261659325No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9060.19960035866377915No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8820.19431293194420882No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8420.18550055407825833No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT8340.18373807850506824No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA8310.18307715016512194No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7890.17382415340587387No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG7770.17118044004608873No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG7590.167214870006411No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG7400.16302899052008452No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG7100.15641970712062164No Hit
GTCGATCAGGTTATCGTCATCGAACGGTTCATCAGATTCGTCCA6940.15289475597424143No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6860.1511322804010513No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6590.1451839253415347No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6580.14496361589488596No Hit
GCTAGAGTTGGAAGTGGTGCGGGAGCTGGAGCCACCAGAGGAAG6250.1376934041554768No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6160.13571061913563792No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT6060.1335075246691503No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5990.13196535854260893No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5980.1317450490959602No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG5750.12667793182303866No Hit
CAGTTCACCGAACAGCAGATCCGTATCGTCGTGCGGGGTAGAAA5480.12072957676352206No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5310.11698431617049307No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5250.11566245949060051No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5070.11169688945092276No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5030.1108156516643277No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5000.11015472332438142No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT4900.1079516288578938No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT4700.10354543992491855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)