FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007930305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453907
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT54041.1905522496899146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27110.5972589098647961No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20450.45053281839672005No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC20020.44105951219082323No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG19240.42387537535221975No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17570.38708369776187634No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16520.36395120586375623No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15880.3498514012782354No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG15780.34764830681174774No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG15560.34280149898547496No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14420.317686222067516No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG13950.30733167807502415No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC13750.3029254891420489No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12950.2853007334101479No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG12400.27318371384446594No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10820.2383748212739614No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9760.21502201992919254No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9380.20665026095653954No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG9230.2033456192568081No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT8870.19541447917745267No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8740.19255045637101872No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8310.18307715016512194No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG8260.18197560293187812No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7980.17580693842571277No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7910.17426477229917142No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7790.17162105893938626No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7690.16941796447289864No Hit
GCTCTGGCCGATTTCTCCCGTGACGAACACC6920.15245413708094388No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6680.1471667103613736No Hit
GGTGCCCCGCGTGGCTCTCCAGCAACCCCGA6670.14694640091472483No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6620.145844853681481No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6440.14187928364180327No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6300.13879495138872058No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6090.13416845300909658No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5490.1209498862101708No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5490.1209498862101708No Hit
CTGGAGGTTACTGGCGACATGCAATGGGCGG5430.11962802953027823No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5200.11456091225735669No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5060.11147658000427399No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5000.11015472332438142No Hit
GGTATGAACACCGCGAACACCGCTGGTACTT4880.10751100996459627No Hit
GTTGGCGAAGGTCAAGGTCTGCAGGTACCGG4620.10178296435172844No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4570.10068141711848462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)