FastQCFastQC Report
Wed 31 May 2023
EGAF00007930306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426257
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23120.5423957847026559No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA21700.5090825487909876No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18230.42767626103500944No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG16640.39037482082405683No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG15120.35471558238339784No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG14970.3511965785899117No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG13850.32492135026521557No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13050.30615333003328976No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG12830.3009921244695102No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG12810.30052292396371205No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG12130.2845701067665751No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG11540.27072869184552983No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG10960.2571218771773836No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG10190.23905765770415502No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9180.2153630321613487No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8960.21020182659756909No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT8540.200348615975808No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8130.19073000560694606No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG7790.1827535970083776No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG7690.18040759447938684No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7610.17853079245619427No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG7160.16797378107573602No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG7100.16656617955834158No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7090.16633157930544248No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6810.15976277222426846No Hit
GTCGATCAGGTTATCGTCATCGAACGGTTCATCAGATTCGTCCA6500.1524901643843972No Hit
GCTAGAGTTGGAAGTGGTGCGGGAGCTGGAGCCACCAGAGGAAG6180.14498295629162689No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6130.14380995502713154No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6060.142167753256838No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT6000.14076015173944356No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5930.13911794996915006No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5740.1346605451640677No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5640.13231454263507694No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5610.13161074187637975No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG5320.12480733454230664No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5000.11730012644953633No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4970.1165963256908391No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4930.11565792467924281No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT4790.11237352113865579No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG4790.11237352113865579No Hit
CAGTTCACCGAACAGCAGATCCGTATCGTCGTGCGGGGTAGAAA4750.1114351201270595No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4320.10134730925239936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)