FastQCFastQC Report
Wed 31 May 2023
EGAF00007930518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473179
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG47511.0040597744194057No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG32740.6919157443589001No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC29140.6158345995912752No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG25200.5325680133733746No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24660.5211558416582308No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT24530.5184084669860666No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG24150.5103776794828173No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA23880.5046715936252454No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA22200.46916705940035375No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18230.38526646364272293No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT16270.343844507047016No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG15820.3343343639510629No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA15070.31848412545780774No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT13920.2941804264348164No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13330.28171157215345566No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12970.2741034576766932No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG12320.260366584315872No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11220.2371195678590977No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT10690.22591873265719736No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10420.2202126467996255No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10210.21577458002151406No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT9920.2096458211374554No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9740.20584176389907413No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT9700.2049964178461005No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9500.20076968758123248No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9410.19886765896204184No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9370.19802231290906824No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9260.19569761126339083No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC9120.19273890007798317No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8550.18069271882310922No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA7970.16843520105499188No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7940.16780119151526168No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG7870.16632183592255784No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG7750.16378579776363703No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG7130.15068293394254606No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG6910.14603353065119118No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6770.14307481946578354No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6720.14201813689956655No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6530.13800274314794192No Hit
GTGCTGGGAACGCGGGGTCCAAGGGCCAGCGTAGCCCGGATAGC6420.13567804150226445No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6350.13419868590956066No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6290.13293066683010024No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6130.1295492826182058No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG6090.12870393656523219No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5840.12342052373414712No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5800.12257517768117351No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5660.11961646649576588No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5430.11475572669116761No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5350.11306503458522038No Hit
CGGTGCCTGTGGCGGAGGCGGTTCCTGATAGCCCTGGTATGGTG5310.11221968853224679No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5250.11095166945278637No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG4970.10503424708197111No Hit
TGGGTCTTGAGACCACGGTGCCCAAGGGTGGCGATAGCACGGGT4900.10355489148926728No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4860.10270954543629365No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT4810.10165286287007665No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4760.10059618030385964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)