FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007930519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473179
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAGTGATTGACGTTGAAACCACTGAAG46750.9879981994129072No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42930.9072676513539274No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC34930.7381984407592054No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC26960.5697632397042134No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC26260.5549696837771751No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25920.5477842423268996No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25820.5456708771944655No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG24980.5279186100820197No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24930.5268619275158027No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20740.438311928466817No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC19300.407879470559767No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17800.3761789935732566No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17440.3685708790964941No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16260.3436331705337726No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT16190.34215381494106883No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13630.28805166755075773No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA13040.275582813269397No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12820.2709334099780421No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12150.2567738635907342No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12150.2567738635907342No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11250.23775357739882794No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11040.23331551062071645No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10940.23120214548828247No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT10800.2282434343028748No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10790.2280320977896314No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10530.22253734844530296No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10440.2206353198261123No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10150.21450656094205364No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC9870.20858913857123837No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9570.2022490431739363No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9390.19844498593555504No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9090.19210489053825297No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8820.1863988046806811No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8540.18048138230986582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8400.1775226711244582No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG8250.17435262342580715No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA8150.1722392582933731No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC7410.15660035631336133No Hit
GAACCGATCTACGCTACCATCGACGACCTGG7380.15596634677363114No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7150.15110560696903286No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7020.14835823229686862No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6830.144342838545244No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6730.14222947341280995No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC6520.1377914066346985No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA6470.13673472406848147No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6390.13504403196253426No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC6290.13293066683010024No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6150.1299719556446926No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6040.12764725399901516No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5870.12405453327387732No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5570.11771443787657525No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5550.11729176485008844No Hit
CCGCCTGCGCCGCAGGCGCCGTACCAGGGCT5430.11475572669116761No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5250.11095166945278637No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5170.10926097734683914No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4980.1052455835952145No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4930.10418890102899749No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4890.10334355497602388No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT4840.10228687240980686No Hit
TCCCACGAGGAACAGCCACGTTATGAAGACC4770.10080751681710305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)