FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007930619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458670
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68031.483201430222164No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT38020.8289183944884121No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18560.40464822203327017No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16050.3499247825233828No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13430.2928031046286001No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11660.2542132687989186No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11430.24919877035777357No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11270.24571042361610745No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA10790.2352453833911091No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10680.23284714500621365No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10510.22914077659319337No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10030.218675736368195No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9970.2173676063400702No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9400.20494037107288465No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8820.19229511413434494No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8270.18030392220986766No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8040.1752894237687226No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7740.16874877362809865No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7360.1604639501166416No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG7180.1565395600322672No Hit
ATGGAACGTCAGCTGCCAAAAAACGTTCGCC7010.15283319161924694No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6640.14476638977914405No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA6550.14280419473695685No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6520.14215012972289445No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6410.139751891337999No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6300.13735365295310353No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6300.13735365295310353No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6240.13604552292497873No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6180.13473739289685394No Hit
CGTGTCCGTGGCCACTACCGTGCCCCATGGG6130.1336472845400833No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6040.1316850894978961No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG5990.13059498114112542No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5870.12797872108487582No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5870.12797872108487582No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT5620.12252817930102251No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5400.11773170253123161No Hit
GCGCTGTCTTGCGTGGGTGCAGCTGTTGCCC5370.1170776375171692No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5180.11293522576144069No Hit
GCTGAAGAGACTACCATCGAAAACGGCATCT5180.11293522576144069No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5130.11184511740467001No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4880.1063945756208167No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4790.10443238057862951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)