FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007930623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458933
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66731.4540248794486341No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA44890.9781384210767149No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21570.47000324666127735No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20940.4562757526697797No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19270.4198869987558097No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG16210.35321059936853527No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13150.2865341999812609No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11870.258643418538218No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11590.25254231009755235No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11250.24513382127674413No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11210.244262234356649No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10600.2309705338251989No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10590.2307526370951751No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9750.21244931177317822No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9620.20961665428286919No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9480.20656610006253637No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9120.19872181778168052No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8440.18390484014006403No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG8300.1808542859197312No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7530.16407623770790072No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC7450.16233306386771054No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7400.1612435802175917No Hit
ATGTGCGAAGACACCATCACCTACGAATGTC7260.15819302599725887No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7050.15361719466675963No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7020.15296350447668833No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7010.15274560774666454No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6680.14555501565588005No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6670.14533711892585627No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6590.1435939450856661No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6040.1316096249343586No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA5850.12746958706390693No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5750.1252906197636692No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA5720.12463692957359788No Hit
GTAGGTCCAACCCCTCACCAGACCCCGCAGG5620.12245796227336016No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5390.1174463374828134No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5230.11395998980243303No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4990.10873046828186249No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA4930.10742308790171987No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4620.10066828927098291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)