FastQCFastQC Report
Sun 21 May 2023
EGAF00007930633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514138
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT47000.9141514535008111No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG41720.8114552902139114No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39200.7624412122815275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33200.6457410267282325No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23660.46018773169849336No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23460.45629772551338355No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23320.45357472118380665No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22700.4415157020099662No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20960.40767264819951066No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG19670.3825821083055522No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC18670.363132077380003No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17740.3450435486192423No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17310.3366800353212562No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16930.3292890235695475No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14510.2822199487297185No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG14150.2752179375965208No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG13000.2528504020321392No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG12590.24487588935266408No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11560.22484235749934842No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11230.2184238472939172No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10920.21239433770699695No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA10710.20830983121263164No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10510.20441982502752176No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10490.20403082440901082No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC9520.18516429441122811No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC9360.18205228946314023No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9110.17718978173175295No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8980.17466127771143156No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8920.1734942758558986No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8910.1732997755466431No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8660.16843726781525584No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG8590.1670757656504674No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8590.1670757656504674No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG8200.15949025358950322No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG8070.15696174956918182No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7990.15540574709513788No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7720.15015423874523962No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7690.14957073781747313No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7580.14743123441566272No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA7350.1429577273027864No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7330.14256872668427542No Hit
CGCCCAGATCCGGCTCTGGCAGCGCTGACCA7080.13770621895288815No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6800.1322602102937344No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6800.1322602102937344No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6680.12992620658266846No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6530.1270087019438361No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC5560.10814217194605338No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5540.1077531713275424No Hit
CCGGCAGTTGCGCCGACCAGCGCGGCTAGCC5260.10230716266838864No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5200.10114016081285569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)