FastQCFastQC Report
Sun 21 May 2023
EGAF00007930635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476082
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68561.4400880520582588No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT43610.9160186690528103No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG38180.8019626871001214No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35070.7366378060922278No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21520.45202297083275567No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21350.44845215740145605No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG20990.4408904348410568No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20830.4375296692586571No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG19230.4039220134346604No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG18190.38207703714906255No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16410.3446885200448662No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15880.3335559840531673No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15830.3325057448086674No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15090.31696220399006897No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13410.2816741653748724No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12830.26949139013867357No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11790.24764641385307573No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG11630.24428564827067606No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10430.21907990640267855No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA10240.2150889972735789No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10130.21277847093567914No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10110.21235837523787918No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9750.20479665267747993No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9510.19975550430388042No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG8480.17812057586718252No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8430.17707033662268265No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8330.17496985813368285No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8310.17454976243588288No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8300.1743397145869829No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG8140.17097894900458324No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7990.16782823127108354No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7890.16572775278208376No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC7860.16509760923538383No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7840.16467751353758386No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7750.16278708289748403No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7710.16194689150188413No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7480.15711579097718462No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG7150.15018421196348528No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA7030.14766363777668554No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6980.14661339853218563No Hit
CGCCCAGATCCGGCTCTGGCAGCGCTGACCA6550.13758134102948652No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6520.13695119748278656No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6240.13106985771358715No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6180.12980957062018728No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6000.12602870933998764No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5890.12371818300208788No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC5660.11888708247738836No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5180.10880478573018933No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4980.10460382875218975No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4830.10145311101869006No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4800.10082296747199013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)