FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007930663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519674
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA45350.8726624768604934No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT37900.7293033709594862No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32900.6330892059252531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32460.6246223594022406No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22820.4391214492162394No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21350.4108344846961749No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20340.3913992233592598No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19590.37696709860412486No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17720.3409830008813218No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17430.33540257930933626No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16090.3096171830801618No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC16020.30827018476968254No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14040.2701693754161263No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG12470.2399581275953771No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12040.23168370940243307No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11760.22629571616051603No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10260.19743146665024613No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9700.186655480166412No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT9690.18646305183634354No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG9380.18049777360422112No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA8820.16972178712038702No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8560.16471865053860688No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8420.16202465391764836No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7910.1522108090841566No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7710.1483622424827873No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG7610.14643795918210262No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7610.14643795918210262No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC7560.14547581753176028No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA7500.1443212475513495No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7040.13546954436820005No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6700.12892698114587223No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA6330.12180713293333897No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6250.12026770629279125No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6030.11603428303128499No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG5830.11218571642991568No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA5790.11141600310964181No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5690.10949171980895717No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5590.1075674365082725No Hit
ATCCCGCTGACCACTACCCCAACCAAATCCC5450.10487343988731397No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5440.1046810115572455No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5240.10083244495587619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)