FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007930665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480571
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66451.3827301272860826No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA41460.8627237182435061No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT38750.8063324670027947No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29520.6142692755076774No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA19990.4159635100744739No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19630.40847242134877054No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19250.40056516102719475No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA18030.3751786936789777No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16260.3383475074442694No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16170.3364747352628436No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14560.30297292179511454No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13890.28903117333338885No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12270.2553212740677236No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11940.24845444273582884No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG11520.23971483922250822No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10880.22639734815459112No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9250.19247936309098967No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8810.18332358798179665No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG8670.18041038681068977No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT8500.17687292824577427No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7980.16605246675309165No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA7850.16334735137992096No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7410.15419157627072796No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7320.1523188040893021No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7250.15086220350374865No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7160.1489894313223228No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC7100.14774091653470559No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA6900.1435792005759815No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG6800.14149834259661945No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6760.14066599940487462No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5880.12235444918648856No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5810.12089784860093514No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5750.11964933381331791No Hit
GTTACCCTGGATGCAGGTAACCGTTTCTCTA5490.11423910306697657No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG5350.11132590189586972No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA5200.10820461492682663No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5130.10674801434127319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)