FastQCFastQC Report
Wed 31 May 2023
EGAF00007930669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450005
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63461.4102065532605192No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10880.24177509138787345No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA10450.2322196420039777No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8360.18577571360318218No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7180.15955378273574738No Hit
CAGCGTCCGCGCGGCGCTCCGACCCCGACCC6450.143331740758436No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG6270.13933178520238665No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6000.13333185186831256No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5520.12266530371884757No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5270.11710980989100121No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT4950.10999877779135787No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4780.10622104198842236No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4730.10510994322285308No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4650.10333218519794224No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA4590.10199886667925913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)