FastQCFastQC Report
Thu 1 Jun 2023
EGAF00007930977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007930977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31252377
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATACT821071626.272292824318612No Hit
AGATCTGG796457725.48470793117592No Hit
CACCGCTC781183824.995980305754024No Hit
GTTGAGAA678607721.71379476191523No Hit
CACCGCTA701520.2244693259651898No Hit
AGATCTGA538370.17226529681246328Illumina PCR Primer Index 9 (100% over 8bp)
TCGATACA340140.10883652145883176No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)