FastQCFastQC Report
Wed 31 May 2023
EGAF00007931100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45274981
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCTTA1197420726.447735008436556No Hit
GCCGAGAT1152833925.46293503690261No Hit
TAATGACC1147501625.345159173009925No Hit
CGTATCGG924149320.41192021703996No Hit
TAATGCCC1358460.30004650913050634No Hit
CGTATCGA935140.20654674598317335No Hit
TAATGACA829970.18331758107198323No Hit
ATGCCTAA767400.16949758631593903Illumina PCR Primer Index 3 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)