FastQCFastQC Report
Wed 31 May 2023
EGAF00007931166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504733
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA25260.5004626208312117No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19100.37841789619462174No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11370.22526761673993972No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11160.22110700112732867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10240.20287954225303279No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9970.19753017932253292No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9910.19634143200464405No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT9380.18584083069662574No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8940.17712335036544075No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8930.17692522581245926No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8810.17454773117668154No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8340.1652358771865521No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6820.13512094513336753No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6790.1345265714744231No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6540.12957345764988618No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5570.11035537601068288No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5480.10857225503384958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)