FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475058
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29550.6220293101052924No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT20330.4279477453279389No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20260.42647424104004145No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT18230.38374261669101456No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17350.3652185627860177No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17260.36332405727300665No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15360.323328940887218No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13400.2820708208260886No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12570.2645992699838757No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10340.21765763338371316No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG10090.2123951180697936No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9750.20523809724286296No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9730.20481709601774942No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9620.2025015892796248No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9150.19260806048945603No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT9030.19008205313877463No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA9030.19008205313877463No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8440.17766251699792446No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8390.17661001393514056No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8030.1690319918830964No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7620.1604014667682683No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG7450.15682295635480298No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT7450.15682295635480298No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7040.1481924312399749No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6990.14713992817719102No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6940.1460874251144071No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC6880.14482442143906638No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT6200.13051037978520516No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6170.12987887794753483No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5850.12314285834571778No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5840.12293235773316101No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5830.12272185712060422No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5730.1206168509950364No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5550.11682783996901432No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5460.11493333445600326No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5330.11219682649276509No Hit
TTGACCACGCTGCTGCGGCTGCTGCGGTTCTTCGAAAGTGCTCG5130.10798681424162944No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4900.10314530015282344No Hit
TTCTGCGGAGTTCGGTACGATTTCCAGCTGCGGAACTTTATATT4900.10314530015282344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)