FastQCFastQC Report
Wed 31 May 2023
EGAF00007931172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475058
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44340.9333597160767738No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43620.9182036719726854No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT29360.6180297984667136No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27880.5868757078083097No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20600.43363126186697204No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG19970.4203697232758948No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18670.39300464364351295No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17560.36963907564971016No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12440.26186276202063746No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11140.23449768238825575No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11130.23428718177569896No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10600.2231306493101895No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10400.21892063705905382No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10370.21828913522138352No Hit
TCCTACACCAACGGCAAACTGACTATCACCC10010.21071111316933933No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9540.20081758437917055No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9520.20039658315405698No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG9450.1989230788661595No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8660.18229353047417368No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8230.17324200413423202No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8020.1688214912705396No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG7990.16818998943286925No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7610.16019096615571152No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7500.1578754594175869No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7490.15766495880503012No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG7310.15387594777900804No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7280.15324444594133768No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7120.14987643614042917No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG6920.14566642388929352No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT6430.13535189387401117No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6170.12987887794753483No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6060.12756337120941022No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5920.12461636263361527No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5350.11261782771787866No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5340.11240732710532186No Hit
CTGTCCCGTTGCACCCTGAACCGCAACGCAC5290.11135482404253796No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA5140.10819731485418621No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4920.103566301377937No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4910.10335580076538023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)