FastQCFastQC Report
Sun 21 May 2023
EGAF00007931198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493992
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA27720.5611426905698877No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27560.5579037717209996No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT20880.4226789097799155No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20000.4048648561110302No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19830.4014235048340864No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17850.3613418840790944No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15520.3141751283421594No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14960.30283891237105054No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14200.2874540478388314No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12910.2613402646196699No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12350.25000404864856113No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11990.24271648123856257No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11680.2364410759688416No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10780.21822215744384524No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA10220.20688594147273642No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10150.2054689144763478No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9660.19554972550162755No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9630.194942428217461No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG9480.1919059417966283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8870.17955756368524187No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8270.16741161800191096No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8200.16599459100552236No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7950.16093378030413447No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7840.1587070235955238No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7670.15526567231858007No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7480.15141945618552527No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7430.1504072940452477No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7220.1461562130560819No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7070.14311972663524913No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7020.14210756449497158No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6960.1408929699266385No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6440.13036648366775172No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6400.12955675395552962No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6230.1261154026785859No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6150.12449594325414177No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6080.12307891625775316No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6070.12287648382969765No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG6060.12267405140164213No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6050.12247161897358662No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC5830.11801810555636529No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5180.10485999773275681No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4970.10060891674359099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)