FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441280
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49091.1124456127628717No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT39660.8987490935460479No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37840.8575054387237128No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24600.5574691805656272No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22900.5189448875997099No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21570.48880529369108044No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21550.4883520667150109No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21160.4795141406816534No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG20290.4597987672226251No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18190.4122099347353155No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16430.37232596084118924No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15600.3535170413343002No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14860.33674764321972445No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC12850.29119833212472807No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12140.27510877447425675No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9540.21618926758520668No Hit
ATCAACTCCAACGCCCCGATGGATAAATGCG9080.2057650471356055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8710.19738034807831764No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8680.1967005076142132No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8570.1942077592458303No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8440.1912617839013778No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8360.18944887599709934No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8210.1860496736765772No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8070.1828770848440899No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7710.17471899927483683No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7680.1740391588107324No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7010.15885605511240028No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6860.15545685279187818No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6730.15251087744742567No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6380.1445794053662074No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6300.14276649746192893No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5940.13460841189267586No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5890.1334753444525018No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA5710.12939630166787527No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5590.12667693981145758No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA5460.12373096446700507No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5310.12033176214648296No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5230.1185188542422045No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5220.11829224075416969No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5150.11670594633792604No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG5090.1153462654097172No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5070.11489303843364757No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC4700.10650833937635969No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4700.10650833937635969No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4580.10378897751994198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)