FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393700
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT83782.128016256032512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50951.2941325882651766No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA23510.5971551943103887No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20740.5267970535941072No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC15230.38684277368554737No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT13270.33705867411734824No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11620.2951485902971806No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10590.2689865379730759No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG10290.26136652273304545No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8480.21539243078486156No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7920.20116840233680466No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7110.18059436118872238No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6960.17678435356870714No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC6900.17526035052070105No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6830.17348234696469395No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5890.14960629921259844No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5520.14020828041656083No Hit
GTTACCATGACCTTCAAGCTGGGCCCTCGTA5490.13944627889255778No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5450.13843027686055373No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5360.1361442722885446No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5230.13284226568453136No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG4900.12446024892049784No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4870.12369824739649479No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG4780.12141224282448565No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4590.11658623317246636No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4340.11023622047244094No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC4290.10896621793243587No Hit
GGTCGCTGGAACCCGCAGCCGGGCGTGTACC4260.10820421640843282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)