FastQCFastQC Report
Sun 21 May 2023
EGAF00007931219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408063
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27580.6758760289465107No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT23440.5744211065448227No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17780.43571703388937494No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17490.428610288117276No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14700.3602384925857037No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12500.3063252487973671No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12490.30608018859832914No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10800.26466501496092515No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10800.26466501496092515No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10060.24653056023212103No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8870.2173683965466117No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8780.21516285475527064No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7850.19237225624474652No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7290.17864888509862448No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT7130.17472792191401817No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7080.1735026209188287No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG7000.17154213932652557No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6560.16075949056885824No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6510.15953418957366877No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6250.15316262439868356No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6210.15218238360253195No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6130.1502219020102288No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG5660.1387040726554478No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA5560.13625347066506888No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5290.12963684529104574No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG5080.12449058111124998No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5070.12424552091221208No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG4910.12032455772760578No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4810.11787395573722684No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4600.11272769155743108No Hit
CAGACGGTCGGTTGGATCCGGATACGCGCCGCGCAGCGGTGCTT4420.10831660797474901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)