FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404734
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA30870.7627231712680427No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA27730.685141352097921No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT16300.40273364728438926No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14860.3671547238433144No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11690.2888316771015037No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11660.28809044952981466No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA11070.2735129739532631No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT9130.2255802576507039No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8070.19939021678435712No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8060.19914314092712745No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC7900.19518992721145248No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6720.16603497605834944No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6680.16504667262943068No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6450.1593639279131479No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC6320.15615194176916197No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5860.14478645233659637No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5750.14206861790706984No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5740.14182154204984015No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT5670.14009201104923233No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC5460.1349034180474089No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5210.12872652161666676No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5200.12847944575943707No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG5190.12823236990220738No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5090.1257616113299105No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4950.1223025493286949No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT4930.12180839761423554No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4720.1166198046124121No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4650.11489027361180429No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC4590.11340781846842618No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4570.1129136667539668No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4570.1129136667539668No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4500.11118413575335899No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4430.10945460475275119No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4240.10476016346538713No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4210.10401893589369808No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG4190.10352478417923872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)