FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460293
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT49111.0669291081984735No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC44490.9665582574577497No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT40740.885088411077292No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA37590.8166537401177075No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC32980.716500142300665No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA30910.6715287870986524No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA25740.5592090255554614No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT24340.5287936162400906No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC22590.4907743545958769No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT19080.4145185783837686No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18690.40604571436020104No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC17890.3886654804657034No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA16900.3671574410212626No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG16310.35433951852407053No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT15990.3473874249662715No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12940.2811252832434993No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA12430.270045384135757No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12280.2667865902805387No Hit
GCGCAGCGGTGCGTCCTTATCACCGGTCGGCGCCGGAATGTCGG9430.2048695070313909No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9200.19987268978672282No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9050.1966138959315045No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8890.19313784915260498No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8860.1924860903815613No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8650.1879237789842557No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8340.18118893835013786No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8220.1785819032659632No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7780.1690227746239895No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7590.1648949690740463No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7000.1520770465768543No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7000.1520770465768543No Hit
GTCTTCCGGGCCTTCCTCACCCGGGCGACGGTGCGCGCGGTGAC7000.1520770465768543No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6690.14534220594273647No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC6520.14164890624015572No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6420.13947637700334353No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG6340.13773835361389375No Hit
GTTGCACGCAGCGTTCCAGGTCTTTTCGAACGCATCAGTGCTGT6070.1318725246745008No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5990.13013450128505102No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5940.12904823666664494No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5940.12904823666664494No Hit
ACGAACGATTACTTTCTGGCGGTCCACGATGTACAGGCCTTTGC5870.1275274662008764No Hit
GTCTGGGCCATCTTCACCCGGGCGACGATGTACACGGTGACGGC5660.12296515480357077No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5430.11796833755890271No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC5370.11666482001681537No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5150.11188525569582854No Hit
TTTCACGACCACTTTAACGGTTTCGTCACCAACAGTTACCAGTA5070.11014723230637875No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4640.10080535658808629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)