FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460293
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA82591.7942918966831995No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC59151.285051043574419No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT49121.0671463611221548No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA38190.8296889155385808No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37340.8112224170256772No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT35930.7805897547866251No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG32050.6962956203983115No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA27870.6054838982995614No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA25150.5463911030582694No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC20540.44623750524122674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20220.4392854116834277No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19190.416908360544262No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG18270.39692109156558975No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA15490.33652477878221043No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13960.30328508145898375No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13890.3017643109932152No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13410.29133617065651657No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10150.22051171753643875No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10120.2198599587653951No Hit
GTTGTGAACAAAATTGATCTGCCGTCTGCTG9570.20791104796292798No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9380.2037832424129848No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9380.2037832424129848No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9310.20226247194721622No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8980.19509312546573596No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8880.19292059622892374No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8870.19270334330524252No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8750.19009630822106788No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8600.18683751436584958No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8530.18531674390008102No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8450.1835787205106313No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7300.15859463428729093No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC7280.1581601284399285No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC7020.15251155242421677No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6900.1499045173400421No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6780.14729748225586745No Hit
ACCAAACAGTATCATCGCTCCTCTCGTAACC6470.14056264162174964No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6350.137955606537575No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6150.13361054806395056No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6140.13339329514026935No Hit
ACCGCCACCGTTAAAGCATATGGCGGCGAAG6140.13339329514026935No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA5840.12687570742983273No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5730.12448592526933933No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC5500.11948910802467125No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5140.1116680027721473No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5030.10927822061165388No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4910.10667118552747923No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG4770.10362964459594215No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4760.10341239167226093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)