FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447116
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG32950.7369452222689414No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG25530.570992762504585No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20630.46140151549038727No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG17630.3943048336449601No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17350.3880424766727203No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG16450.36791347211909214No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15080.3372726540763471No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14400.3220640728580503No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13980.3126705373996905No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13350.29858023421215074No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA13010.29097594360300233No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11730.2623480260156201No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA11540.25809856949874305No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA11430.2556383578310774No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11270.25205986813265463No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10070.22522119539448376No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9190.20553950205315843No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8550.19122554325946733No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7840.17534599522271627No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7660.17132019431199064No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA7580.16953094946277925No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7070.15812451354905663No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG6760.15119118975836246No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5550.12412886141404021No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5450.12189230535252597No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5350.11965574929101172No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5230.11697188201719463No Hit
AACGCTCTGGGTGTGTTTCGGACCAGAGGAAACGCTGGCCAGGG4670.10444716807271491No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT4660.10422351246656349No Hit
ACGGCGATAGTTGGTCGCTTCCATGAACTGGGAAGTGCCCAGCG4660.10422351246656349No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4560.10198695640504926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)