FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417784
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG26940.6448308216686134No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG24900.5960017616758899No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23000.5505237156042356No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21840.5227581716868047No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA20690.4952319859065929No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG20190.48326407904563123No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG19470.4660302931658465No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG19280.46148248855868107No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16100.385366600922965No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG16070.38464852651130726No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16040.3839304520996496No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG15490.3707657545525918No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG14690.3516171035750531No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14550.34826608965398387No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13780.32983551308810294No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA12770.3056603412289604No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG12450.29800088083794496No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG12020.28770848093751794No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG10850.2597035788828677No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC10660.2551557742757023No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG10620.25419834172682537No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9160.21925205369281733No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG8830.21135323516458265No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT8440.2020182678130326No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8130.19459816555923634No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8020.1919652260498248No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG7650.18310897497271317No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7610.18215154242383624No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG7480.1790398866399862No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG7300.17473144017004003No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG7100.16994427742565538No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7020.1680294123279015No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6830.16348160772073608No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG6810.1630028914462976No Hit
CGGGCACTGCGGAACGGTCAGGTACAGGCCGCGACGTTTTTCCG6730.16108802634854374No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6660.1594125193880091No Hit
CGGGGTCAGGCGAGGCTGGGACAGCGGAGACAGAATGGTCGGCG6460.15462535664362445No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG6460.15462535664362445No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC6320.1512743427225552No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6240.14935947762480134No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG6100.14600846370373205No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5840.13978515213603201No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG5630.13475863125442814No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5520.13212569174501657No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5510.13188633360779733No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT5320.1273385290006319No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5240.12542366390287804No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG5240.12542366390287804No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG4980.11920035233517799No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4890.11704612910020488No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4880.11680677096298567No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT4840.11584933841410873No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4640.11106217566972407No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4540.10866859429753174No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA4240.10148785018095476No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT4180.10005170135763936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)