FastQCFastQC Report
Wed 31 May 2023
EGAF00007931246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417784
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49921.1948758209984107No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC30060.7195105604810141No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26120.6252034544166363No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG25050.5995921337341784No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23700.567278785209582No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG22280.5332899297244509No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21730.5201252321773931No Hit
ATGTCCAACATGGACATCGATGGTATTAACA20930.5009765811998546No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG19900.4763226930662735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19710.4717748884591081No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17180.41121727974264216No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG16480.3944622101372959No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC16310.39039312180456887No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16040.3839304520996496No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG15540.37196254523868794No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13950.3339046014208299No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13840.3312716619114183No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG12670.3032667598567681No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12350.2956072994657526No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC12230.2927350018191219No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10840.25946422074564846No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG10490.2510866859429753No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9640.23074124427934053No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8830.21135323516458265No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8720.20872029565517108No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8610.20608735614575951No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8330.19938532830362102No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8140.19483752369645557No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8110.19411944928479788No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7830.18741742144265935No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC7660.1833483331099324No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7440.1780824540911093No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7010.16779005419068227No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6960.16659326350458611No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6870.164439040269613No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA6820.16324224958351685No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6600.1579763705646937No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6550.15677957987859756No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6530.15630086360415907No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6440.154146640369186No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG6440.154146640369186No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT5800.1388277195871551No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5540.13260440801945503No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5530.1323650498822358No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5490.13140761733335884No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5490.13140761733335884No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5340.12781724527507035No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5210.12470558949122035No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5200.12446623135400112No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4940.11824291978630105No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4750.11369511517913564No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG4600.11010474312084714No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4460.10675372919977788No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4340.10388143155314708No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4310.10316335714148939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)