FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007931248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398596
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51191.2842577446838401No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT48181.208742686830776No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC28780.7220343405352788No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26110.6550492227719295No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG22220.5574566729219561No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21980.5514355387409808No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG21450.5381388674246605No Hit
ATGTCCAACATGGACATCGATGGTATTAACA20620.5173157783821212No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20070.5035173458840531No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG18420.46212204838984833No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17330.43477606398458585No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15440.387359632309406No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG15420.38685787112765807No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15200.3813384981284308No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15070.3780770504470692No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13000.32614476813615795No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13000.32614476813615795No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11710.2937811719134161No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11710.2937811719134161No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11320.28399682886933136No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG10300.25840700860018667No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10090.25313851619183336No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG9070.22754869592268864No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8490.2129976216519985No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8390.21048881574325884No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8160.20471856215315756No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8090.2029623980170398No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7680.19267629379120715No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7360.18464811488324018No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7270.18239018956537448No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7040.1766199359752732No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG6990.17536553302090338No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6920.1736093688847856No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA6810.17084968238517195No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6490.162821503477205No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6430.16131621993196119No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6400.16056357815933928No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6270.15730213047797773No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT5900.1480195486156409No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5830.1462633844795231No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG5770.14475810093427932No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5570.13974048911679998No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5400.1354755190719425No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5180.12995614607271522No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5100.1279491013457235No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5040.12644381780047967No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5010.1256911760278578No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT4870.12217884775562224No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA4860.12192796716474827No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4650.11665947475639495No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4380.10988569880279783No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4260.10687513171231021No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4250.10662425112143623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)