FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510645
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA22050.431806832535323No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18680.36581186538593347No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17830.3491662505262952No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16180.31685417462229143No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13260.2596715918103575No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC13070.25595080731232067No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC11710.22931782353689942No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG11660.22833866972162659No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8750.1713519176727472No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8560.16763113317471043No Hit
ATCGGAACCGATCTGCAGGCCGGATTTGGTAATGGTTTCGCCAG8300.16253953333529164No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7810.1529438259456178No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7670.15020219526285383No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7560.1480480568692536No Hit
ATCGGAGCCGATCTGCAGGCCGCTTTTGGTAATGGTTTCACCGG7280.1425647955037257No Hit
ATCGGAACCAATTTGCAGACCGGATTTGGTGATGGTTTCGCCAG7170.14041065711012543No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7160.14021482634707086No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6800.1331649188771064No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6670.13061911895739703No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6440.12611501140714196No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6100.11945676546328662No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA6090.11926093470023207No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5730.1122110272302676No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5590.10946939654750364No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5370.10516111976030314No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG5320.1041819659450303No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5310.10398613518197573No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5270.10320281212975746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)