FastQCFastQC Report
Wed 31 May 2023
EGAF00007931320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495236
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA102342.0664895120710125No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA54081.0920046200195461No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA50251.014667754363576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40370.8151669103215436No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC22880.4620019546236542No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG22200.44827112730092317No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20970.42343448376127746No Hit
GAATGCGACAACTATCGCCAGGAATACGATA20270.4092998085761132No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG19850.40081900346501464No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG18910.38183815393065124No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14000.2826935037032849No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13670.27603001397313603No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA12720.256847240507556No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT12010.2425106413911751No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA11900.24028947814779214No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10020.20232777907906535No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9390.18960657141241752No Hit
TCCTACACCAACGGCAAACTGACTATCACCC9340.18859695175633434No Hit
ATCGAAGGTACTGACGATAAATGCGAGGAAG9270.18718348423781794No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG9240.18657771244416804No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9090.18354885347591854No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG9010.18193346202618552No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8540.17244303725900378No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8090.16335646035425536No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7960.16073144924843913No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7800.157500666348973No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7730.1560871988304566No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7550.1524525680685572No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7080.14296214330137552No Hit
ATGCAGCAGCAGGCATACATTAACGCGACCA6890.1391255886082595No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6570.1326640228093273No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6450.1302409356347277No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6390.1290293920474279No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6360.128423620253778No Hit
GTTGAAACCCCGCAGCCGACCGCTGTATCCG5940.11994281514267945No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5660.11428894506861376No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5400.10903892285698133No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5260.10621198781994848No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5030.10156773740196594No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5000.10096196560831602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)