FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540574
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT63511.174862276025114No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10740.1986777018502555No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10320.1909081827834857No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9990.18480356065959516No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9620.17795898433886942No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6890.12745711040486593No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6270.11598782035392009No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA6260.11580283180471129No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5940.10988319823002955No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5930.10969820968082075No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5930.10969820968082075No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5760.10655340434427109No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5560.10285363336009501No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5490.10155871351563339No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5470.10118873641721576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)