FastQCFastQC Report
Wed 31 May 2023
EGAF00007931351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529624
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA33450.6315801398728154No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA31410.5930622479343837No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG23240.43880186698487983No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22870.43181577874114463No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG16770.31663972931740253No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC14420.27226862830989534No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG14010.26452728728305364No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13510.25508662749422234No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG12290.23205141760947387No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12240.23110735163059076No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT11450.21619110916423728No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10640.20089724030633052No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA10160.19183420690905245No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9750.18409286588221077No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG8970.16936543661163392No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8930.1686101838285274No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8820.1665332386749845No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7910.1493512378593115No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7870.148595985076205No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7550.14255396281135296No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT7530.1421763364197997No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7340.13858888570004382No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG7330.13840007250426717No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7090.13386855580562815No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG7040.132924489826745No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6790.12820415993232936No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6760.12763772034499946No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6380.12046281890548767No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6340.11970756612238115No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6310.1191411265350513No Hit
ACGCATTGCGCCGTTAGACGGGTTCAGATAGTACCAAGTGGAAC6300.11895231333927465No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6100.11517604942374213No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5820.1098892799419966No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT5690.10743470839690045No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG5650.10667945561379394No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT5620.10611301602646406No Hit
CAGATTTTCTTCGTGTGCGTCCACGTTGTTATGGTTGTAGGAGT5420.10233675211093153No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5310.10025980695738863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)