FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552257
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA34810.6303224766729983No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA33320.6033422844798708No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG25350.4590254175139473No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22780.41248911285868717No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG18350.3322728367408652No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG15080.2730612740082969No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15050.27251804866212653No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13390.24245957950736705No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG13270.24028667812268562No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12250.22181701635289366No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT11890.21529831219884946No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11590.20986605873714592No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA10270.18596414350565044No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9680.1752807116976335No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG9420.17057275869749047No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9420.17057275869749047No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9400.17021060846671024No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8460.15318954762003922No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT8050.1457654678890444No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7750.1403332144273409No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7730.13997106419656066No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7660.13870353838882984No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG7530.1363495618887583No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG7520.13616848677336818No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7300.13218483423478564No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6910.12512290473457105No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6850.12403645404223033No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6850.12403645404223033No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6570.11896635081130706No Hit
ACGCATTGCGCCGTTAGACGGGTTCAGATAGTACCAAGTGGAAC6550.11860420058052681No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6540.11842312546513671No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT6310.11425839781116401No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6060.10973151992641109No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5990.10846399411868027No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG5820.10538571715704825No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT5810.10520464204165814No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5710.10339389088775697No Hit
CAGATTTTCTTCGTGTGCGTCCACGTTGTTATGGTTGTAGGAGT5630.10194528996463603No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5590.10122098950307556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)