FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552257
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT72001.3037408308088443No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA32210.5832429466715677No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC26380.47767615439912936No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23100.41828351655117096No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC19600.3549072261646299No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16650.3014900671245453No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC15610.2826582551239731No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15280.2766827763160992No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12700.22996539654544895No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12630.22869787073771813No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC11650.21095250942948662No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11510.208417457814025No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10930.19791510112139818No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA10710.1939314485828156No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10570.19139639696735397No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG10370.18777489465955163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9590.1736510356591225No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG8780.158983951312523No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8330.1508355711199677No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA7880.14268719092741242No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7700.13942783885039028No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG7490.13562526142719783No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7390.13381451027329666No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7100.12856333192698327No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6900.12494182961918093No Hit
GCTATGCAGACCGGTTGGTACAAAGTAGGCA6710.1215014024267687No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6660.1205960268498181No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6550.11860420058052681No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6480.117336674772796No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6380.11552592361889483No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC6310.11425839781116401No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6230.11280979688804307No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6030.10918829458024072No Hit
CGCGATAACAACATTGAACGTGACAACAATA5930.10737754342633955No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC5870.10629109273399885No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5590.10122098950307556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)